SIRT1 antibody (Internal Region)

Catalog No. ABIN185702

The Goat Polyclonal anti-SIRT1 antibody has been validated for WB and ELISA. It is suitable to detect SIRT1 in samples from Mouse.

Quick Overview for SIRT1 antibody (Internal Region) (ABIN185702)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SIRT1 Antibody

Binding Specificity: Internal Region

Purpose: SIRT1

Sequence: EITEKPPRTQ KE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EITEKPPRTQKE, from the internal region of the protein sequence according to NP_036370.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 80.3 kDa according to Mouse NP_062786.1). Preliminary testing also showed a band at approx 120x kDa in Fetal Mouse Brain lysate. This molecular weight is routinely o

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIRT1 , sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , RP11-57G10.3, SIR2L1 , OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtui

Gene ID: 23411, 93759, 309757

NCBI Accession: NP_036370

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling